Abstract
1. A 'cavity' organ culture method was developed, which allowed the insertion within bovine nasal cartilage of 10 microliter of 35S-labelled proteoglycan aggregate. 2. Stimulation of the tissue with retinol or catabolin led to degradation of both the cartilage matrix and the proteoglycan within the cavity. 3. Proteoglycan aggregate trapped in polyacrylamide beads was not broken down in the cavities of living cartilage, except under autolytic conditions. 4. It is suggested that degradation of proteoglycan aggregate by chondrocytes occurs in a pericellular region.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dingle J. T., Blow A. M., Barrett A. J., Martin P. E. Proteoglycan-degrading enzymes. A radiochemical assay method and the detection of a new enzyme cathepsin F. Biochem J. 1977 Dec 1;167(3):775–785. doi: 10.1042/bj1670775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dingle J. T. Heberden oration 1978. Recent studies on the control of joint damage: the contribution of the Strangeways Research Laboratory. Ann Rheum Dis. 1979 Jun;38(3):201–214. doi: 10.1136/ard.38.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dingle J. T., Saklatvala J., Hembry R., Tyler J., Fell H. B., Jubb R. A cartilage catabolic factor from synovium. Biochem J. 1979 Oct 15;184(1):177–180. doi: 10.1042/bj1840177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dingle J. T. The secretion of enzymes into the pericellular environment. Philos Trans R Soc Lond B Biol Sci. 1975 Jul 17;271(912):315–324. doi: 10.1098/rstb.1975.0055. [DOI] [PubMed] [Google Scholar]
- Hardingham T. E., Muir H. Hyaluronic acid in cartilage and proteoglycan aggregation. Biochem J. 1974 Jun;139(3):565–581. doi: 10.1042/bj1390565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hascall V. C., Heinegård D. Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid. J Biol Chem. 1974 Jul 10;249(13):4232–4241. [PubMed] [Google Scholar]
- Kobayashi I., Ziff M. Electron microscopic studies of the cartilage-pannus junction in rheumatoid arthritis. Arthritis Rheum. 1975 Sep-Oct;18(5):475–483. doi: 10.1002/art.1780180507. [DOI] [PubMed] [Google Scholar]