Table 1.
Presentation of the basic data of the studied population and individual results of the histopathological analysis in the control (Co.) and RIC groups.
| Group | Age (weeks) | Weight (g) | Tumor diameter (mm) | Necrosis (%) | Average number of CD3+ cells/HPF, tumor center | CD3+ cells in the tumor periphery | Average number of Tregs/HPF, tumor center | Tregs in the tumor periphery | MAb1 | CA IX |
|---|---|---|---|---|---|---|---|---|---|---|
| Co.1 | 9.5 | 25.3 | 10.5 | 20.52 | 3.1 | +/++ | 1.8 | + | ++ | +++ |
| Co. 2 | 9.5 | 25.1 | 11 | 11.03 | 0.8 | + | 0.96 | +/++ | + | +++ |
| Co. 3 | 9.5 | 19.4 | 8.35 | 6.49 | 1.7 | + | 1.0 | + | + | +++ |
| Co. 9 | 11.5 | 22 | 8.5 | 43.09 | 1.2 | + | 0.75 | + | +++ | +++ |
| Co. 13 | 9 | 22.5 | 8.5 | 9.24 | 1.8 | +/++ | 2.45 | + | ++ | +++ |
| Co. 16 | 14 | 23.3 | 8.5 | 47.81 | 1.5 | ++/+++ | 0.67 | + | ++ | +++ |
| Co. 18 | 9.5 | 23 | 9.5 | 54.41 | 1.8 | + | 0.2 | -/+ | ++ | +++ |
| Co.19 | 14.5 | 26.6 | 10.0 | 31.36 | 3.7 | ++/+++ | 0.22 | + | +++ | +++ |
| Co. 20 | 10 | 20 | 7.25 | 30.92 | 1.9 | ++/+++ | 2.75 | ++ | ++ | +++ |
| Co. 22 | 10.5 | 24.5 | 8.7 | 36.73 | 1.8 | ++ | 1.18 | +/++ | +++ | +++ |
| Co. 24 | 11 | 20.1 | 7.5 | 8.42 | 1.5 | + | 0.21 | -/+ | ++ | +++ |
| Co. 26 | 11.5 | 20.5 | 8.5 | 11.1 | 1.5 | ++ | 0.35 | + | + | +++ |
| Co. 27 | 12 | 22.2 | 9.25 | 9.66 | 1.1 | +/++ | 0.0 | -/+ | ++ | +++ |
| Co. 29 | 13.7 | 29 | 9.0 | 32.23 | 1.0 | +/++ | 0.12 | -/+ | + | +++ |
| RIC 4 | 9.5 | 19.8 | 9.5 | 14.65 | 2.9 | + | 0.0 | -/+ | ++ | +++ |
| RIC 6 | 10 | 22 | 8,0 | 24.69 | 1.4 | ++ | 0.27 | + | +++ | +++ |
| RIC 7 | 12 | 26.8 | 10 | 36.62 | 2.2 | ++ | 0.74 | + | +++ | +++ |
| RIC 8 | 13 | 28 | 9.0 | 32.7 | 5.5 | ++/+++ | 1.25 | +/++ | +++ | +++ |
| RIC 10 | 12 | 22.8 | 9.5 | 23.59 | 1.8 | -/+ | 0.4 | -/+ | +++ | +++ |
| RIC 11 | 12 | 24.8 | 15.0 | 3.7 | +/++ | 1.21 | ++ | +++ | ++ | |
| RIC 12 | 12 | 24.8 | 8.75 | 4.7 | ++/+++ | 5.33 | ++ | ++ | ++ | |
| RIC 14 | 9 | 19 | 7.75 | 39.84 | 3.1 | + | 0.25 | -/+ | +++ | +++ |
| RIC 15 | 9 | 21.5 | 8.5 | 27.1 | 2.8 | ++/+++ | 0.44 | -/+ | +++ | +++ |
| RIC 17 | 14 | 27.1 | 8.0 | 6.45 | 4.5 | +++ | 0.0 | +/++ | + | +++ |
| RIC 21 | 10 | 21.6 | 9.0 | 24.86 | 4.1 | ++/+++ | 1.1 | +/++ | ++ | +++ |
| RIC 23 | 11 | 20.4 | 7.5 | 6.86 | 2.0 | + | 0.3 | + | + | +++ |
| RIC 25 | 11.5 | 26.7 | 9.25 | 31.74 | 2.0 | +/++ | 0.41 | + | + | +++ |
| RIC 28 | 13.5 | 28 | 7.2 | 60.13 | 1.1 | +/++ | 0.59 | + | + | +++ |
Two RIC samples were not considered for necrosis area measurement because the samples were partially fragmented.
The symbols “+”, “++”, “+++”, and “-” represent a semi-quantitative grading system for hypoxia and T-cell infiltration. For hypoxia, + indicates a few small areas, ++ moderate, and +++ large hypoxic areas. For T-cell infiltration, - means no detectable T cells, + a few, ++ moderate, and +++ strong infiltration. FoxP3-positive cells at the tumor edge are classified as - (negative), + (low), and ++ (mild). In cases of borderline findings, two values can be indicated (e.g., +/++, ++/+++, -/+).