Fig. 6.

PLA2G4D enhances the incorporation of MAG from extracellular sources into cellular acylglycerols independently of DGAT. (A) Representative TLC of acylglycerol levels of control (empty vector transfection) and PLA2G4D-expressing COS7 cells loaded with 300 μM rac-18:1 MAG for 210 min in the absence and presence of DGAT1 and DGAT2 inhibitors (5 μM each). (B, C) Densitometric quantification of acylglycerol bands in rac-18:1 MAG loaded cells in the absence and presence of DGAT inhibitors (shown in A). Experiments were performed in triplicates (n = 3) and data are presented as mean ± SD and are representative for two independent experiments. Statistical comparison in all graphs was performed with an unpaired two-tailed Student’s t test. Statistically significant differences are shown as: ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. DGAT, diacylglycerol acyltransferasel; FC, free cholestero; MAG, monoacylglycerol; PLA2G4, phospholipase A2 group IV.