Fig. 7.

PLA2G4D mRNA expression in mouse tissues and keratinocytes. (A) Pla2g4d mRNA expression normalized to 36b4 housekeeping (HK) gene in murine tissues. Ct values for Pla2g4d are shown above each bar. Tissues were analyzed in 2–4 biological replicates. (B) Pla2g4d mRNA expression normalized to 36b4 HK gene in primary keratinocytes and the human HaCaT keratinocyte cell line (n = 3). Primary cells were isolated from the epidermis of two-day-old neonatal mice and cultured for four days in the presence of low CaCl₂ concentrations (0.06 mM). Keratinocyte differentiation was induced by increasing CaCl₂ to 0.2 mM for 24 h. Before RNA isolation, primary and HaCaT keratinocytes were cultured in absence and the presence of IL17A and/or TNFα (20 ng/ml each) for 24 h. Ct values above 30 were considered as no or negligible expression for Pla2g4d (nd). Data are shown as mean ± SD. Statistical comparisons in (B) were performed with one-way ANOVA followed by Bonferroni posthoc analysis. Statistically significant differences are shown as: ∗P < 0.05; ∗∗P < 0.01; and ∗∗∗P < 0.001. BAT, brown adipose tissue; CM, cardiac muscle; iWAT, inguinal white adipose tissue; gWAT, gonadal white adipose tissue; SM, skeletal muscle; SI, small intestine; PLA2G4, phospholipase A2 group IV.