Fig. 5.

PDZ domain-containing 8 (PDZD8) knockdown inhibits palmitic acid (PA)-induced insulin 1 (INS-1) cell death. (A, B) T he apoptosis of INS-1 cells was detected using the flow cytometry. (C) The cytotoxicity was detected by lactate dehydrogenase (LDH) kit. (D) The expression of mitochondrial apoptosis-related proteins was detected by Western blotting. PE-H, phycoerythrin-H; FITC-H, fluorescein isothiocyanate-H; siPDZD8, siRNA PDZ domain-containing 8; Bcl-2, B cell lymphoma 2; Bax, BCL2 associated X, apoptosis regulator; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. ^aP<0.05 vs. control (Con) group, ^bP<0.05 vs. PA+shRNA negative control (shNC) group.