Fig. 2.

Agarose gel electrophoresis (2%). (A) Polymerase chain reaction amplification product of ACE2 SNP (rs2285666). Numbers refer to lanes. Lane 1 shows 50-bp DNA ladder; lanes 2–6 are the amplified DNA segments with 466-bp bands. (B) ACE2 single nucleotide protein (rs2285666) restriction products using the restriction fragment length polymorphism-polymerase chain reaction method. Lane 1 displays 50-bp ladder of DNA; lanes 2, 17, 18, and 20 refer to homozygous genotype AA (with 281- and 185-bp DNA fragment sizes), lanes 3–7, 11–16, and 19 refer to homozygous genotype GG (with 466-bp DNA fragment size), and lanes 8–10 refer to heterozygous genotype GA (with 466-, 281-, and 185-bp DNA fragment sizes, respectively). ACE2, angiotensin-converting enzyme 2; SNP, single nucleotide polymorphism.