Fig. 3.

Agarose gel electrophoresis (2%) of the restriction fragment length polymorphism-polymerase chain reaction restriction products for the TNF-α-308G/A promoter single nucleotide protein. Numbers refer to lanes. Lane 1 shows 50-bp DNA ladder; lane 2 is the no template control; lanes 3 and 6–8 refer to homozygous genotype GG (with 87-bp DNA fragment sizes); lane 5 refers to homozygous genotype AA (with 107-bp DNA fragment size); and lane 4 refers to heterozygous genotype GA (with 87-, 20-, and 107-bp DNA fragment sizes). The 20 bp-fragment could not be visualized on the gel. TNF, tumor necrosis factor.