FIGURE 7.

DRA’s role in HD’s intestinal barrier protection. Post-DRA siRNA transfection, Caco-2 cells were treated with TNF-α (10 ng/mL) and TNF-α (10 ng/mL) + HD (10 μM) for 24 h (A) TEER and (B) FITC-dextran flux were measured, (C, D) Cytokine levels (IFN-γ/IL-13) in the supernatants via ELISA (n = 4). (E) WB images for ZO-1, E-cadherin, occludin, and GAPDH in Caco-2 cells. (F) Densitometric analysis of ZO-1, E-cadherin, and occludin. (G) Representative WB images of HNF-1β and GAPDH in Caco-2 cells. (H) Densitometric analysis of HNF-1β. Data, presented as mean ± SEM from 3-4 independent experiments. ^## P < 0.01 compared with the control group and ^* P < 0.05, ^** P < 0.01 compared with the TNF-α (10 ng/mL) group, ^$ P < 0.05, ^$$ P < 0.01 compared with the TNF-α (10 ng/mL) + HD (10 μM) group.