Table 3.
Relationship between clinicopathological characteristics and CD8+, CD56+, CD57+, GNLY+, and GZMB+ TIL subsets
| Clinicopathological characteristics | CD8+ TIL | CD56+ TIL | CD57+ TIL | GNLY+ TIL | GZMB+ TIL | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of TILs | p value | No. of TILs | p value | No. of TILs | p value | No. of TILs | p value | No. of TILs | p value | |
| Age (year) | 0.990 | 0.027 | 0.306 | 0.052 | 0.473 | |||||
| < 50 | 198.2 (113.7-332.3) | 2.3 (0.1–6.9) | 7.7 (1.4–15.7) | 17.8 (6.1–36.5) | 7.7 (1.0–31.0) | |||||
| ≥ 50 | 220.3 (80.3-377.3) | 0.7 (0.0-4.3) | 5.7 (0.7–13.7) | 11.3 (2.0–30.0) | 5.3 (1.3–22.3) | |||||
| Histologic type | 0.422 | 0.755 | 0.779 | 0.938 | 0.979 | |||||
| IC-NST | 200.0 (89.1-362.2) | 1.7 (0.0–6.0) | 6.7 (1.0-15.7) | 13.4 (3.3–34.1) | 7.5 (1.0-25.5) | |||||
| Othersa | 255.3 (144.2-331.9) | 1.7 (0.2–6.4) | 5.0 (0.7–13.4) | 10.7 (1.0-48.4) | 7.3 (1.5–31.4) | |||||
| T stage | 0.044 | 0.066 | 0.098 | 0.005 | 0.024 | |||||
| T1-2 | 217.3 (112.4-362.5) | 2.0 (0.0–6.0) | 6.7 (1.1–15.7) | 14.7 (4.3–35.7) | 7.7 (1.1–26.0) | |||||
| T3-4 | 70.0 (5.7-256.7) | 0.0 (0.0-0.3) | 0.7 (0.0-8.7) | 1.3 (0.0–7.0) | 0.3 (0.0-9.7) | |||||
| N stage | 0.540 | 0.343 | 0.374 | 0.083 | 0.125 | |||||
| N0 | 221.7 (87.2-373.3) | 2.0 (0.0–6.0) | 6.9 (1.1–15.8) | 13.9 (4.7–39.9) | 8.5 (1.1–26.0) | |||||
| N1-3 | 188.3 (118.3-294.7) | 1.0 (0.0–5.0) | 5.7 (1.0-12.3) | 12.0 (2.0-19.3) | 2.3 (0.3–20.0) | |||||
| AJCC stage | 0.410 | 0.087 | 0.161 | 0.147 | 0.030 | |||||
| I-II | 219.8 (102.3-365.2) | 2.0 (0.0–6.0) | 6.9 (1.0-15.9) | 14.2 (3.6–36.5) | 8.2 (1.3–26.0) | |||||
| III | 153.3 (115.7-294.7) | 0.3 (0.0–4.0) | 3.3 (0.5-8.0) | 10.7 (2.0-16.7) | 1.0 (0.0-7.3) | |||||
| LVI | 0.169 | 0.001 | 0.002 | 0.001 | 0.021 | |||||
| Absent | 232.3 (117.3–374.0) | 2.7 (0.0-7.3) | 8.3 (1.3–18.3) | 20.3 (4.7–46.0) | 9.7 (1.3–31.7) | |||||
| Present | 170.3 (86.4-315.8) | 0.6 (0.0-3.3) | 5.0 (0.4–8.5) | 11.0 (2.0-19.2) | 2.3 (0.4–20.0) | |||||
| Histologic grade | 0.335 | 0.066 | 0.080 | 0.009 | 0.041 | |||||
| II | 144.7 (67.5-288.5) | 0.0 (0.0-3.7) | 1.3 (0.2–10.7) | 1.3 (0.3–17.2) | 1.3 (0.0-4.5) | |||||
| III | 217.3 (109.3-362.2) | 2.0 (0.0–6.0) | 6.9 (1.2–15.8) | 14.7 (4.7–36.7) | 8.2 (1.0–26.0) | |||||
| Nuclear pleomorphism | 0.104 | 0.516 | 0.505 | 0.032 | 0.382 | |||||
| 2 | 127.3 (46.0-292.3) | 0.0 (0.0-7.3) | 7.3 (0.3–11.7) | 0.7 (0.3–22.7) | 1.7 (0.0-39.3) | |||||
| 3 | 219.8 (110.6–357.0) | 1.7 (0.0–6.0) | 6.5 (1.1–15.6) | 14.0 (4.7–35.7) | 7.7 (1.0-24.7) | |||||
| DCIS component | 0.157 | 0.006 | 0.066 | 0.002 | 0.020 | |||||
| Absent | 254.7 (113.8-369.1) | 3.0 (0.5–9.2) | 8.7 (1.6–18.7) | 24.5 (9.6–56.7) | 14.5 (2.7–35.3) | |||||
| Present | 179.3 (103.7-331.3) | 1.0 (0.0–5.0) | 6.0 (0.7–12.7) | 11.3 (2.4–27.7) | 3.7 (0.7–22.3) | |||||
| Tumor border | 0.229 | 0.038 | 0.011 | < 0.001 | 0.002 | |||||
| Pushing | 234.0 (120.7–366.0) | 2.0 (0.0-7.7) | 8.0 (2.0–17.0) | 21.3 (6.7–46.3) | 14.3 (1.0–35.0) | |||||
| Infiltrative | 185.5 (87.4-319.7) | 1.4 (0.0–4.0) | 3.7 (0.7–10.3) | 6.9 (1.5–17.8) | 2.2 (1.0-15.4) | |||||
| Multicentricity | 0.524 | 0.951 | 0.645 | 0.994 | 0.911 | |||||
| Absent | 217.3 (86.3–347.0) | 1.9 (0.0–6.0) | 6.7 (1.0-15.7) | 13.0 (2.5–36.0) | 7.3 (1.0-23.4) | |||||
| Present | 190.3 (140.3-345.2) | 1.0 (0.2–4.2) | 6.3 (0.9–13.0) | 14.3 (5.8–31.5) | 8.7 (1.0-32.5) | |||||
| Ki-67 index | 0.028 | 0.011 | 0.111 | 0.003 | 0.015 | |||||
| < 50% | 154.6 (77.8–302.0) | 0.9 (0.0-4.1) | 5.9 (0.7–13.2) | 11.0 (1.3–22.7) | 2.7 (0.7–20.1) | |||||
| ≥ 50% | 250.3 (122.9-377.8) | 3.0 (0.3-7.0) | 8.0 (2.0-16.5) | 21.3 (6.5–42.7) | 14.7 (1.3–36.7) | |||||
| p53 overexpression | 0.568 | 0.815 | 0.629 | 0.339 | 0.831 | |||||
| Absent | 192.3 (84.5-362.4) | 1.7 (0.0–6.0) | 6.7 (0.9–16.5) | 11.7 (1.3–32.8) | 8.7 (0.7–22.4) | |||||
| Present | 221.7 (109.3-334.5) | 1.7 (0.0-5.4) | 6.5 (1.2–15.0) | 14.5 (5.2–36.0) | 6.8 (1.2–26.6) | |||||
| Basal phenotype | 0.385 | 0.515 | 0.397 | 0.844 | 0.552 | |||||
| Absent | 155.5 (86.3-292.3) | 2.0 (0.0–7.0) | 7.0 (0.3–13.0) | 16.7 (0.7–34.3) | 7.3 (0.5–22.7) | |||||
| Present | 219.8 (107.6–357.0) | 1.7 (0.0-5.3) | 6.5 (1.1–15.6) | 13.0 (4.3–35.3) | 7.5 (1.0-27.7) | |||||
Data are presented as median (inter-quartile range), and p values were calculated by Mann-Whitney U test
aInclude metaplastic carcinoma and carcinoma with apocrine differentiation
TIL, tumor-infiltrating lymphocyte; GNLY, granulysin; GZMB, granzyme B; IC-NST, invasive carcinoma of no special type; AJCC, American Joint Committee on Cancer; LVI, lymphovascular invasion; DCIS, ductal carcinoma in situ