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. 2024 Dec 5;15:465. doi: 10.1186/s13287-024-04070-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — T₂ values in Day 1 iPSCs could predictively indicate Day 10 T₂ and OCT4 levels. A Differentiating Day 1 iPSC with lower T₂ values resulted in Day 10 SCPCs with lower T₂. B Differentiating Day 1 iPSC with lower T₂ values resulted in Day 10 SCPCs with higher levels of OCT4⁺ cells as quantified by immunofluorescent staining. At least 10 ROIs (more than 60,000 cells) were quantified for each experimental repeat (n = 11)