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. Author manuscript; available in PMC: 2025 Feb 1.
Published in final edited form as: Nat Chem Biol. 2023 Nov 9;20(2):243–250. doi: 10.1038/s41589-023-01476-2

Fig. 2 |. Steps and intermediates in F-ring modification of AFEs.

Fig. 2 |

a, Late-stage intermediates and shunt metabolites resulting from TnmK1, TnmJ, TnmK2 and DynA4. For the structures of S1 and S2, see Supplementary Fig. 9. b, Metabolite profiles, upon HPLC analysis with detection at 539 nm and 460 nm, of crude extracts from the ΔtnmJ and ΔtnmK2 mutants and their respective complemented strains, with Streptomyces sp. (S. sp.) CB03234 wild-type as a control. Full traces shown in Supplementary Fig. 4. c, UV–Vis profiles of selected compounds highlighting different levels of conjugation.