Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (E.coli) | nfuA, nadK, fabG, sodA, tpiA, gpmA, can, upp, speB, ispD, acuL, thiD, pyrF, nadE, crp | Extracted by qPCR from BL21 E. coli K12 | Marciano et al., 2022 | |
| Cell line (Human) | HeLa cell line | Epithelial cell | RRID: CVCL_0030 | |
| Peptide, recombinant protein | nfuA, nadK, fabG, sodA, tpiA, gpmA, can, upp, speB, ispD, acuL, thiD, pyrF, nadE, crp | Genes were cloned into pET-His- bdSumo and purified as described | Marciano et al., 2022 | |
| Chemical compound, drug | Dulbecco’s phosphate-buffered saline (PBS 1 X) | Biological Industries | Cat# 02-023-1A | |
| Chemical compound, drug | HEPES buffer | Fisher BioReagents | LOT# 170358 | |
| Chemical compound, drug | DMEM(1 X) Gibco | Life Technologies Limited | REF# 41965–039 | |
| Chemical compound, drug | Fetal Bovine Serum (Gibco) | Life Technologies Limited | REF# 12657–029 | |
| Chemical compound, drug | Trypsin-EDTA Solution A | Biological Industries | REF# 03-050-1B | |
| Chemical compound, drug | Penicillin/ Streptomycin | Biological Industries | REF# 03-031-1B | |
| Chemical compound, drug | Sodium Pyruvate Solution | Biological Industries | REF# 03-042-1B | |
| Chemical compound, drug | 35 mm glass-bottomed dishes | MatTek Corporation | P35G-0–14 C | |
| Chemical compound, drug | Pierce Dye Removal Columns | Thermo-Fisher scientific | Cat# 22858 | |
| Chemical compound, drug | Gebaflex tubes 3.5 KDa (GeBa) |
TIVAN BIOTECH | Cat# MIDI3-100 | |
| Chemical compound, drug | Capillary Glass Tubing | Warner Instruments | Model No. G120TF-4 203-776-0664 |
|
| Chemical compound, drug | well Black F-Bottom Plate | Greiner-bio-one | REF# 655076 | |
| Chemical compound, drug | Disposable cuvettes | Fisher Scientific | ZEN0040 | |
| Chemical compound, drug | BSA (Albumin Bovine, fraction V) |
MP Biomedicals, LLC | CAS# 9048-46-8 | |
| Chemical compound, drug | HEWL (Lysozyme from chicken egg white) | Merck | CAS# 12650-88-3 | |
| Chemical compound, drug | Myoglobin (from equine heart) |
Merck | CAS# 100684-32-0 | |
| Chemical compound, drug | Doxorubicin | AdooQ Bioscience | Cat# A14403 | |
| Chemical compound, drug | Fluorescein disodium salt | chemcruz | Cat# sc-206026 | |
| Chemical compound, drug | GSK3 inhibitor SB216763 | Abcam | Cat# ab120202 | |
| Chemical compound, drug | Quinacrine dihydrochloride | Abcam | Cat# ab120749 | |
| Chemical compound, drug | Primaquine biphosphate | Merck | CAS# 63-45-6 |
|
| Chemical compound, drug | CCF2-FA | Thermo-Fisher Scientific | Cat# K1039a | |
| Chemical compound, drug | CF514 Labelling dye | Biotium | CF514 Dye Alternative green fluorescent dye | |
| Chemical compound, drug | Amodiaquine | AdooQ Bioscience | Cat# A17660 | |
| Chemical compound, drug | Sodium Azide | Merck | CAS# 26628-22-8 |
|
| Chemical compound, drug | Mitoxantrone | Abcam | Cat# ab141041 | |
| Chemical compound, drug | Bafilomycin A1 | Abcam | Cat# ab120497 | |
| Chemical compound, drug | 5-amino Fluorescein | Abcam | Cat#ab145305 |