Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 4;25(12):5667–5686. doi: 10.1038/s44319-024-00305-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) Depiction of MPP7 domain architecture and V5-tagged wild type (WT) and domain deletion mutants (listed on the right) of Mpp7 expression constructs used in (B, C). (B) Flowchart to force-express various Mpp7 constructs (A) in Mpp7 cKO MuSCs. (C) Quantifications of cells fate fractions from experiments depicted in (B). IF of V5, PAX7 and MYOD was performed to determine the fate of transfected cells. Expression constructs used are in x-axis; (−), empty vector with IRES-GFP; keys to cell fate at the top. (D) Co-IP assays to determine interaction domains between MPP7 and AMOT in 293T cells. HA-tagged AMOT WT and AMOT △PDM, and V5-tagged MPP7 WT and MPP7 △PDZ were used in co-IP using an anti-V5 antibody, followed by Western blotting with anti-HA or anti-V5 antibodies. (E–H) Amot cKO regenerative defects at 5 dpi using the same experimental design as in Fig. 1B (Con, Pax7^CreERT2/+): Representative H&E staining images of TA muscles from Con (a different biological replicate compared to Fig. 1C, Con) and Amot cKO (E), quantifications of regenerated muscle fiber cross-sectional areas (F), PAX7⁺ MuSC densities (G), and percentages of EdU⁺ YFP-marked cells (H). (I) SM transfection assays (as depicted in B) with Amot WT or △PDM constructs; Con (−) from Fig. 2C; IF of PAX7, MYOD and HA was performed to determine transfected cells’ fates; keys at the top. (J, K) IF of MPP7 in Con and Amot cKO MuSCs (J) and of AMOT in Con and Mpp7 cKO MuSCs (K), at 48 h after FACS isolation. Qualified fluorescent signals (arbitrary units, AU) are to the right. Data information: Scale bars = 25 µm (E, J, K). (C) ≥ 168 transfected cells were assessed per group; (F–H) N = 5 mice in each group; (I) ≥180 cells per group; (J, K) 200 cells per group. Bars represent means ± SD (F–H) or medians ± 95% CI (J, K). Student’s t test (two-sided) were used in (F–H). Chi-square tests were performed in (C, I). Mann–Whitney test were performed in (J, K). Source data are available online for this figure.