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. 2024 Nov 4;25(12):5667–5686. doi: 10.1038/s44319-024-00305-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) H&E histology of Yap cKO and YapTaz cKO muscles at 5 dpi (Con (Pax7^CreERT2/+) histology not included); quantifications of regenerated myofiber cross-sectional area to the right. (B) Venn diagram shows overlapping DEGs between the YapTaz cKO and the Mpp7 cKO. (C, D) IF images of TAZ (C) and YAP (D) in FACS-isolated and cultured Con (Pax7^CreERT2/+), Mpp7 cKO, and Amot cKO MuSCs at 48 h; relative fluorescent signals (in AU) to the right. (E) Co-IP of V5-TAZ and HA-AMOT by FLAG-MPP7 expressed in 293 T cells; tagged epitopes used for IP and Western blotting are indicated; (−), empty expression construct. Quantification of relative levels of co-IPed V5-TAZ is to the right. (F) Relative TEAD-reporter (8XGTIIC-luciferase, depicted at top) activities when co-transfected with V5-Taz, Flag-Mpp7, and/or Ha-Amot constructs in 293T cells; (−), empty expression construct. (G) Relative activities of WT and TEAD-binding site mutated (MUT) Carm1-reporters co-transfected with V5-Taz or Flag-Mpp7 constructs; (−), empty expression construct. (H) Relative IF signals (AU) of CARM1 in Mpp7 cKO MuSCs transfected with empty vector (−), V5-Taz WT and V5-Taz S89A constructs (all with IRES-mGFP) (I) Relative cell fate fractions among Con (Pax7^CreERT2/+), Mpp7 cKO, and Mpp7 cKO MuSCs transfected with V5-Taz S89A and V5-Taz WWm construct in single myofiber culture; Con (−) and Mpp7 cKO (−) from Fig. 2C; keys at the top. Data information: Scale bar = 50 µm in (A) and 25 µm in (C, D). (A) N = 5 mice in each group; (C, D, H) 200 cells from 2 to 3 mice in each group; (E–G) n = 3 biological experiments; (I) ≥ 150 cells in each group. Bars represent medians ± 95% CI (C, D, H) or means ± SD (A, E, F, G). Hypergeometric test was used in (B), one-way ANOVA with Tukey’s post hoc test performed in (A, E, F, G), Kruskal–Wallis test followed by Dunn’s multiple comparisons test in (C, D, H), and Chi-square test used in (I). Source data are available online for this figure.