Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| E. coli BL21 codon plus (DE3)-RIPL | Agilent Technologies | Cat # 130280 |
| E. coli BL21 (DE3) | In-house | N/A |
| Recombinant DNA | ||
| pET-SUMO-PARP1 | This study | N/A |
| pET-SUMO-mCherry-PARP1 | This study | N/A |
| pET-SUMO-PARP1-ZnF | This study | N/A |
| pET-SUMO-PARP1 ΔZnF | This study | N/A |
| pET-SUMO-Lig3 | This study | N/A |
| pET-SUMO-XRCC1 | This study | N/A |
| pET-SUMO-PARG | This study | N/A |
| pET-SUMO-Polβ | This study | N/A |
| pET28a-His-MBP-FUS-GFP | This study | N/A |
| pET-SUMO-Caspase-3 WT | Genscript | N/A |
| pET-SUMO-Caspase-3 C165S | Genscript | N/A |
| pFl Strep Sumo TEV OAS1 | Ando et al, 2019 | N/A |
| pET24a-PARP5a (1093-1327) | Rhine et al, 2022 | N/A |
| Antibodies | ||
| Mouse Anti-PAR | Enzo Life Sciences | ALX-804-220-R100 |
| anti-Mouse IgG, HRP | Cell Signaling Technology | 7076 V |
| Oligonucleotides and other sequence-based reagents | ||
| DNA substrates and oligos | This study | Appendix Table S2 |
| Chemicals, enzymes, and other reagents | ||
| 18:1 (Δ9-Cis) PC (DOPC) | Avanti Polar Lipids | Cat # 850375 P |
| 18:1 Biotinyl Cap PE | Avanti Polar Lipids | Cat # 870273 P |
| 18:1 PEG2000 PE | Avanti Polar Lipids | Cat # 880130 P |
| 2-mercaptoethanol | Sigma-Aldrich | Cat # M3148-250ML |
| ABT-888 | Toronto Research Chemicals | Cat # A112580-10 |
| Alexa Fluor 488 NHS-ester | Thermo Fisher Scientific | Cat # A20000 |
| Benzamidine | BioShop | Cat # BEN601.5 |
| Bovine Serum Albumin | Thermo Fisher Scientific | Cat # BP9706100 |
| Catalase | Sigma-Aldrich | Cat # C100 |
| Chloramphenicol | BioShop | Cat # CLR201.5 |
| Cy3-dATP | Jena Bioscience | Cat # NU-835-CY3 |
| DNase | Roche Diagnostics | Cat # 4536282001 |
| DTT | Goldbio | Cat # DTT50 |
| EDTA | Wisent Bioproducts | Cat # 625-060-CG |
| Gibson Assembly Master Mix | New England Biolabs | Cat # E2611S |
| Glucose Oxidase | Sigma-Aldrich | Cat # G2133 |
| Glycerol | BioShop | Cat # GLY002.4 |
| Glycine | BioShop | Cat # GLN001.1 |
| HEPES | BioShop | Cat # HEP005.100 |
| Histones | Sigma-Aldrich | Cat # H9250 |
| Imidazole | BioShop | Cat # IMD508.100 |
| isopropyl β-D-1-thiogalactopyranoside (IPTG) | BioShop | Cat # IPT001.5 |
| Kanamycin | BioShop | Cat # KAN201.5 |
| Kapa HiFi Hotstart ReadyMix | Roche Diagnostics | Cat # 07958927001 |
| Lambda DNA | New England Biolabs | Cat # N3011S |
| LB Broth (Miller) | BioShop | Cat # LBL407.5 |
| Luminata Crescendo Western HRP Substrate | Sigma-Aldrich | Cat # WBLUR0500 |
| Lysozyme | BioShop | Cat # LYS702.5 |
| Magnesium chloride | BioShop | Cat # MAN222.100 |
| Methanol | Caledon Laboratories | Cat # 6701-7-40 |
| Nicotinamide adenine dinucleotide (NAD+) | New England Biolabs | Cat # B9007S |
| Ni-NTA Agarose | Qiagen | Cat # 30210 |
| Nt.BspQI | New England Biolabs | Cat # R0644S |
| PMSF | BioShop | Cat # PMS123.25 |
| Poly(I:C) RNA | Invivogen | Cat # Tlrl-picw |
| Potassium chloride | BioShop | Cat # POC888.1 |
| QuickChange Site-Directed Mutagenesis Kit | Agilent | Cat # 200515 |
| RNase A | Thermo Fisher Scientific | Cat # EN0531 |
| SfoI | New England Biolabs | Cat # R0606S |
| Skim milk powder | BioShop | Cat # SKI400.1 |
| Sodium chloride | BioShop | Cat # SOD002.5 |
| Streptavidin | Thermo Fisher Scientific | Cat # 434302 |
| SYTOX Orange Nucleic Acid Stain | Thermo Fisher Scientific | Cat # S11368 |
| T4 DNA Ligase | New England Biolabs | Cat # M0202S |
| Tris | BioShop | Cat # TRS001.1 |
| tris(2-carboxyethyl)phosphine (TCEP) | BioShop | Cat # TCE101.1 |
| Tween-20 | BioShop | Cat # TWN510.500 |
| Zinc chloride | BioShop | Cat # ZNC233.100 |
| Software | ||
| Fiji | https://fiji.sc/ | |
| Matlab | https://www.mathworks.com/products/matlab.html | |
| GraphPad Prism | https://www.graphpad.com/ | |
| Biorender | https://www.biorender.com/ | |
| ImageStudio | https://www.licor.com/bio/image-studio/ | |
| Adobe Illustrator | https://www.adobe.com/ | |
| Other | ||