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. 2024 Nov 7;25(12):5780–5809. doi: 10.1038/s44319-024-00304-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV4 — (A) Crossing scheme to generate nrpd1- and nrpe1-EVD lines. F2 plants were genotyped to select homozygous WT and mutant lines for each background and propagated through selfing until the F6 generation. (B, C) % methylated cytosines by bisulfite -PCR DNA methylation analysis at EVD-GAG sequences in the F6 generation of NRPD1-EVD (B) and NRPE1-EVD (C) lines, in both WT (darker shade) and mutant (lighter shade) backgrounds. Col-0, nrpd1 and nrpe1 were used as controls. Error bars represent 95% confidence Wilson score intervals of the % of methylated cytosines (C) in each context (CG, CHG, CHH). (D, E) Dot-plot representation of bisulfite-PCR sequencing data for the F6 generation of NRPD1-EVD (D) and NRPE1-EVD (E) lines, in both WT and mutant backgrounds, at EVD-GAG sequences. Col-0 and nrpd1 or nrpe1 were used as control for the endogenous parental EVD copies. (F, G) Dot-plot representation of bisulfite-PCR sequencing data for the F6 generation of NRPD1-EVD (F) and NRPE1-EVD (G) lines, in both WT and mutant backgrounds, at EVD-LTR sequences. Col-0 and nrpd1 or nrpe1 were used as control for the endogenous parental EVD copies. In all dot-plots, filled circle represent methylated, empty circles unmethylated cytosines in the CG (red), CHG (blue), and CHH (green) context. Source data are available online for this figure.