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. 2024 Nov 7;25(12):5780–5809. doi: 10.1038/s44319-024-00304-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) RNA blot analysis of EVD siRNAs against GAG and LTR in 10 F6 individual plants of RDR6-EVD and rdr6-EVD lines. tasiR255 probe is used as control for RDR6 mutation, miR171 and snoRNA U6 are shown as loading controls. WT Col-0 and rdr6 with no reactivated EVD are shown as negative control for EVD activity. (B) Analysis of EVD shGAG expression of the same individuals investigated in A by qPCR, normalized to ACT2. (C) EVD copy number analysis by qPCR of the same individuals investigated in (A) and (B), using the EVD-GAG sequence as target. In (B) and (C), qPCR technical replicates for each sample are represented by dots. p-values for two-sided t-test between indicated samples are shown. Differences are considered statistically significant if p < 0.05 (5.00e−2) or non-significant (ns) if p ≥ 0.05. Green and red arrows indicate individuals with active and silenced EVD copies, respectively, selected individuals for subsequent EM-sequencing are indicated by an asterisk in (A–C). Source data are available online for this figure.