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. 2024 Dec 6;9:241. doi: 10.1038/s41541-024-01040-6

Fig. 6. Immunogenicity and identification of candidate antigen peptides of Plasmodium P36 and P52 proteins in C57BL/6 mouse model.

Fig. 6

a. C57BL/6 mice were gene-gun primed with DNA encoding P36 or P52 on days 0 and 2. Finally, mice were sacrificed 14 days post priming and splenocytes were subjected to IFN-γ ELISPOT. b. IFN-γ ELISPOT data for candidate antigens P52 and P36 using respective proteins for in vitro stimulation. c. Screening result of responding 5-peptide pools of P52 protein antigen in mice by IFN-γ ELISPOT (Pool #1, Pool #2, Pool #3). d Screening result of responding individual peptide from the 5-peptide pools (1 and 3) of P52 protein antigen in mice by IFN-γ ELISPOT. N = 5 mice per group. Data is from an experiment. Data are the mean ± SEM.