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. 2024 Jul 26;5(6):745–750. doi: 10.3168/jdsc.2024-0546

Table 2.

Individual AA quantitative range, R2, qualitive control signal, instrument and method repeatabilities, and recovery

AA Standard curve linear range (μmol/L) Standard curve signal range (V/min) R2 QC1 signal range (V/min) QC (μmol/L) Instrument repeatability (%) Method repeatability (%) Total repeatability (%) Recovery (%)
Asp 0.27–52.6 12,592–2,150,700 0.9999 76,042–114,554 4.8 1.11 2.56 2.72 86.2
Glu2 3.81–292.89 70,562–8,592,941 0.9999 707,437–1,037,916 58.8 0.56 2.52 2.56 97.1
Asn 1.64–315.52 4,473–2,906,556 0.9999 87,995–147,824 53.6 1.53 1.94 2.31 94.5
Gln 4.45–855.74 16,060–7,262,293 0.9999 602,215–934,404 273.7 0.50 2.39 2.42 95.4
His 0.93–179.12 28,189–3,697,592 0.9998 446,655–568,939 41.1 1.13 1.99 2.19 97.6
Ser 2.8–538.34 25,254–5,854,739 0.9999 485,735–751,310 110.8 0.66 1.89 1.97 97.6
Gly2 8.24–328.35 13,932–457,164 0.9995 156,011–213,717 296.6 1.18 2.12 2.33 93.7
Thr 2.9–558.25 36,551–10,799,604 0.9998 203,194–373,786 85.9 0.77 2.43 2.51 99.7
Ala 6.14–1,180.91 35,808–4,506,686 0.9999 53,657–71,804 281.9 1.00 1.89 2.06 92.6
Arg 3.08–592.55 8,784–1,190,030 0.9999 56,582–81,641 62.2 2.29 3.34 3.83 90.4
Pro 2.65–508.63 140,316–14,267,622 0.9996 4,258,770–4,647,082 104.6 0.63 1.67 1.75 96.6
Tyr 2.1–403.09 7,743–1,578,059 0.9999 109,304–128,470 97.9 0.53 1.14 1.22 97.0
Val 6.57–1,262.37 57,661–5,073,884 0.9998 1,169,823–1,359,375 224.7 0.33 1.79 1.81 98.6
Met 0.48–93.18 7,212–670,589 0.9998 43,071–53,907 21.3 0.40 1.51 1.54 102.1
Ile 3.27–628.04 34,063–2,536,612 0.9998 819,336–908,762 116.2 0.49 1.68 1.72 98.6
Leu 4.06–779.37 25,759–1,893,897 0.9997 867,042–959,339 199.5 0.55 1.25 1.33 96.1
Trp 1.24–238.14 26,114–341,176 0.9993 98,759–137,102 43.4 1.68 1.65 2.15 91.2
Phe 1.34–258.36 17,766–1,588,804 0.9999 489,834–600,854 49.2 0.38 1.64 1.67 95.7
Lys 2.68–515.99 21,588–592,032 0.9994 46,301–102,596 84 1.62 2.94 3.22 96.7
1

QC = quality control samples pooled from plasma samples from lactating dairy cows consuming 15% to 16.5% CP diets. Signal range and average concentration of 5 independent aliquots.

2

Curve points were removed from Glu (highest and lowest: 366.8 and 1.91 μM, respectively) and Gly (4 highest: 1,652.67 to 664.06 μM) to preserve linearity.