Table 1.
Picolinic LC-MS/MS separation gradient.
| Time (min) | Flow (mL/min) | B |
|---|---|---|
| 0 | 0.25 | 20% |
| 1 | 0.25 | 20% |
| 6 | 0.25 | 60% |
| 26 | 0.25 | 60% |
| 41 | 0.25 | 95% |
| 46 | 0.25 | 95% |
| 47 | 0.25 | 20% |
| 62 | 0.25 | 20% |
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