Table 3.
Girard-P LC-MS/MS separation gradient.
| Time (min) | Flow (mL/min) | B |
|---|---|---|
| 0 | 0.5 | 15% |
| 4 | 0.5 | 25% |
| 8 | 0.5 | 28% |
| 12 | 0.5 | 80% |
| 13 | 0.5 | 100% |
| 18 | 0.5 | 100% |
| 19 | 0.5 | 15% |
| 24 | 0.5 | 15% |
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