Skip to main content
. Author manuscript; available in PMC: 2025 Jul 4.
Published in final edited form as: Biomed Microdevices. 2024 Jul 4;26(3):32. doi: 10.1007/s10544-024-00714-1

Fig. 2.

Fig. 2

The dynamic flow amnion membrane organ-on-chip (AM-OOC) used for modeling the impact of amniotic fluid motion on cells. a Illustration of the dynamic flow AM-OOC and image of a device ready for testing. It consists of two cell culture chambers and a media reservoir. The two bottom cell culture chambers are interconnected by an array of microchannel. The top reservoir block is punched to match with inlets and outlet of the bottom cell culture chamber. Scale bar = 1 cm. b The two amnion membrane cells (hFM_AECs and hFM_AMCs) cultured in the AM-OOC, observed using bright field microscopy. Scale bar = 25 μm. c The experiment flow of creating in vitro amniotic fluid flow that mimics the in vivo condition. Syringe pumps and control units are connected to the AM-OOC device, where the device is incubated for cell co-culture while the dynamic flow condition is applied.