Cardiomyocyte culture medium

Reagent	Final concentration	Amount
DMEM F12 with stable Glutamine and 15 mM HEPES	91%	45.5 mL
Horse Serum (HS)	5%	2.5 mL
Penicillin/Streptomycin (100x)	1%	0.5 mL
Glutamine (100x)	1%	0.5 mL
MEM Non-essential Amino acids (100x)	1%	0.5 mL
Sodium Pyruvate (100 mM)	1%	0.5 mL
Total	–	50 mL

Store at 4°C for up to 2 weeks.