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. 2024 Nov 22;5(4):103461. doi: 10.1016/j.xpro.2024.103461

Pancreatin and collagenase enzyme solution

Reagent Final concentration Amount
Collagenase A 0.45 mg/mL 22.5 mg
Pancreatin 1 mg/mL 50 mg
Cold ADS buffer 50 mL

Use fresh and keep in ice. See “preparation of enzymatic digestion solution (collagenase-pancreatin enzyme solution)” above for details.