Reagent | Final concentration | Amount |
---|---|---|
Collagenase A | 0.45 mg/mL | 22.5 mg |
Pancreatin | 1 mg/mL | 50 mg |
Cold ADS buffer | 50 mL |
Use fresh and keep in ice. See “preparation of enzymatic digestion solution (collagenase-pancreatin enzyme solution)” above for details.