Figure 2.

ECM-mediated integrin activation differentially promotes formation of spatially distinct ventral Sept-7 structures and promotes its association with FAs

(A) TIRFM images showing paxillin (magenta), and Sept-7 (yellow, low, and high contrast), of MEFs plated on 10 μg/mL FN, PLL, 0.1 μg/mL FN, and 0.1 μg/ml FN + MnCl², magenta and cyan insets highlight perinuclear and peripheral FAs respectively; scale bars, 30 μm (main images), 5 μm (insets).

(B) Quantification of Sept-7 anisotropy of MEFs plated on 10 μg/mL FN, PLL, 0.1 μg/mL FN, and 0.1 μg/ml FN + MnCl², n = 2 replicates, 20–22 cells.

(C) Quantification of peripheral Sept-7 structure area (left plot), and roundness (right plot), for MEFs plated on 10 μg/mL FN, PLL, 0.1 μg/mL FN, and 0.1 μg/ml FN + MnCl², n = 3 replicates, 627–1418 structures.

(D) Quantification of colocalization between paxillin and Sept-7 at peripheral FAs of cells plated on 10 μg/mL FN, PLL, 0.1 μg/mL FN, and 0.1 μg/ml FN + MnCl², n = 3 replicates, 2030–3400 FAs. All statistics performed using Kruskal-Wallis and Dunn’s multiple comparisons test, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, ns, not significant, orange horizonal lines show medians.