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. 2024 Nov 9;27(12):111355. doi: 10.1016/j.isci.2024.111355

Figure 3.

Figure 3

Sept-7 promotes the maturation and stabilization of perinuclear FAs

(A) TIRFM images show MEFs transfected with NT (top panels) or Sept-7 KD (lower panels) siRNA and labeled with paxillin (magenta) and F-actin (cyan), magenta and cyan boxes highlight perinuclear and peripheral FAs respectively; scale bar, 20 μm.

(B) ROIs of perinuclear (magenta) and peripheral (cyan) FAs from (A); scale bar, 5 μm.

(C and D) Quantification of perinuclear (C) and peripheral (D) average FA number and FA size for NT and Sept-7 KD MEFs plated on 10 μg/mL FN, n = 3 replicates, 670–3160 FAs.

(E and F) Quantification of perinuclear (E) and peripheral (F) FA formation rate and minimum FA lifetime for NT and Sept-7 KD MEFs plated on 10 μg/mL FN n = 2–3 replicates, 53–75 FAs. All statistics performed using Mann-Whitney test, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, ns, not significant, orange horizonal lines show medians.