| Time (min) | A (%) | B (%) | Flow rate (mL min−1) |
|---|---|---|---|
|
| |||
| 0 | 95 | 5 | 0.5 |
| 0.5 | 95 | 5 | 0.5 |
| 2.5 | 5 | 95 | 0.5* |
| 3 | 5 | 95 | 0.5 |
| 3.25 | 5 | 95 | 0.5 |
*
linear gradient.
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