Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Apr 15;188(1):193–199. doi: 10.1042/bj1880193

Effects of glucose and insulin on the activation of lipoprotin lipase and on protein-synthesis in rat adipose tissue.

S M Parkin, K Walker, P Ashby, D S Robinson
PMCID: PMC1162555  PMID: 6996676

Abstract

Glucose, and certain sugars that can readily be converted to glucose 6-phosphate, bring about an activation of adipose-tissue lipoprotein lipase when epididymal fat-bodies from starved rats are incubated in the presence of cycloheximide. Other substrates do not support the activation. If the tissue is preincubated in the presence of cycloheximide for longer than 2h, the ability of added glucose to activate the enzyme is lost. On the other hand, the addition of glucose still brings about an increase in lipoprotein lipase activity after preincubation in the absence of cycloheximide for as long as 4h. The magnitude of the increase in enzyme activity brought about by the addition of glucose is increased when protein synthesis is stimulated during the preincubation period by insulin. The results are interpreted in terms of the existence in adipose tissue of a proenzyme pool of lipoprotein lipase that is normally maintained by protein synthesis and that is converted to complete enzyme of higher specific activity by a process that specifically requires glucose.

Full text

PDF
194

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashby P., Bennett D. P., Spencer I. M., Robinson D. S. Post-translational regulation of lipoprotein lipase activity in adipose tissue. Biochem J. 1978 Dec 15;176(3):865–872. doi: 10.1042/bj1760865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ashby P., Tolson A. M., Robinson D. S. The heterogeneity of the lipoprotein lipase of rat epididymal adipose tissue. Biochem J. 1978 May 1;171(2):305–311. doi: 10.1042/bj1710305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bensadoun A., Ehnholm C., Steinberg D., Brown W. V. Purification and characterization of lipoprotein lipase from pig adipose tissue. J Biol Chem. 1974 Apr 10;249(7):2220–2227. [PubMed] [Google Scholar]
  4. Cryer A., Foster B., Wing D. R., Robinson D. S. The effect of cycloheximide on adipose-tissue clearing-factor lipase. Biochem J. 1973 Apr;132(4):833–836. doi: 10.1042/bj1320833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Czichi U., Lennarz W. J. Localization of the enzyme system for glycosylation of proteins via the lipid-linked pathway in rough endoplasmic reticulum. J Biol Chem. 1977 Nov 25;252(22):7901–7904. [PubMed] [Google Scholar]
  6. Desai K., Hollenberg C. H. Regulation, by insulin, of lipoprotein lipase and phosphodiesterase activities in rat adipose tissue. Isr J Med Sci. 1975 Jun;11(6):540–550. [PubMed] [Google Scholar]
  7. Iverius P. H., Ostlund-Lindqvist A. M. Lipoprotein lipase from bovine milk. Isolation procedure, chemical characterization, and molecular weight analysis. J Biol Chem. 1976 Dec 25;251(24):7791–7795. [PubMed] [Google Scholar]
  8. Jacobs B. O., Krahl M. E. The effects of divalent cations and insulin on protein synthesis in adipose cells. Biochim Biophys Acta. 1973 Sep 7;319(3):410–415. doi: 10.1016/0005-2787(73)90181-0. [DOI] [PubMed] [Google Scholar]
  9. Jarett L., Steiner A. L., Smith R. M., Kipnis D. M. The involvement of cyclic AMP in the hormonal regulation of protein synthesis in rat adipocytes. Endocrinology. 1972 May;90(5):1277–1284. doi: 10.1210/endo-90-5-1277. [DOI] [PubMed] [Google Scholar]
  10. KRAHL M. E. STIMULATION OF PEPTIDE SYNTHESIS IN ADIPOSE TISSUE BY INSULIN WITHOUT GLUCOSE. Am J Physiol. 1964 Mar;206:618–620. doi: 10.1152/ajplegacy.1964.206.3.618. [DOI] [PubMed] [Google Scholar]
  11. Kneer P., Ball E. G. Studies on the metabolism of adipose tissue. XXI. An evaluation of the major pathways of pyruvate metabolism. J Biol Chem. 1968 Jun 10;243(11):2863–2870. [PubMed] [Google Scholar]
  12. Lingappa V. R., Lingappa J. R., Prasad R., Ebner K. E., Blobel G. Coupled cell-free synthesis, segregation, and core glycosylation of a secretory protein. Proc Natl Acad Sci U S A. 1978 May;75(5):2338–2342. doi: 10.1073/pnas.75.5.2338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miller L. V., Beigelman P. M. Effects of varying concentrations of insulin upon protein synthesis in isolated fat cells. Endocrinology. 1967 Aug;81(2):386–389. doi: 10.1210/endo-81-2-386. [DOI] [PubMed] [Google Scholar]
  14. Minemura T., Lacy W. W., Crofford O. B. Regulation of the transport and metabolism of amino acids in isolated fat cells. Effect of insulin and a possible role for adenosine 3',5'-monophosphate. J Biol Chem. 1970 Aug 10;245(15):3872–3881. [PubMed] [Google Scholar]
  15. Patten R. L. The reciprocal regulation of lipoprotein lipase activity and hormone-sensitive lipase activity in rat adipocytes. J Biol Chem. 1970 Nov 10;245(21):5577–5584. [PubMed] [Google Scholar]
  16. Robinson D. S., Wing D. R. Studies on tissue clearing factor lipase related to its role in the removal of lipoprotein triglyceride from the plasma. Biochem Soc Symp. 1971;(33):123–135. [PubMed] [Google Scholar]
  17. Sakai T., Lavis V., Williams R. H. Incorporation of leucine into protein by isolated fat cells--effects of agents which stimulate glucose metabolism and inhibit lipolysis. Biochem Pharmacol. 1973 Nov 1;22(21):2761–2762. doi: 10.1016/0006-2952(73)90136-6. [DOI] [PubMed] [Google Scholar]
  18. Scow R. O., Blanchette-Mackie E. J., Smith L. C. Role of capillary endothelium in the clearance of chylomicrons. A model for lipid transport from blood by lateral diffusion in cell membranes. Circ Res. 1976 Aug;39(2):149–162. doi: 10.1161/01.res.39.2.149. [DOI] [PubMed] [Google Scholar]
  19. Stark N. J., Heath E. C. Glucose-dependent glycosylation of secretory glycoprotein in mouse myeloma cells. Arch Biochem Biophys. 1979 Feb;192(2):599–609. doi: 10.1016/0003-9861(79)90131-0. [DOI] [PubMed] [Google Scholar]
  20. Vydelingum N., Kissebah A. H., Wynn V. The role of calcium in insulin action. V. Importance of cyclic guanosine 3':5' monophosphate and calcium ions in insulin stimulation of lipoprotein lipase activity and protein synthesis in adipose tissue. Horm Metab Res. 1978 Jan;10(1):38–46. doi: 10.1055/s-0028-1093478. [DOI] [PubMed] [Google Scholar]
  21. WINEGRAD A. I., RENOLD A. E. Studies on rat adipose tissue in vitro. I. Effects of insulin on the metabolism of glucose, pyruvate, and acetate. J Biol Chem. 1958 Aug;233(2):267–272. [PubMed] [Google Scholar]
  22. Wing D. R., Robinson D. S. Clearing-factor lipase in adipose tissue. Studies with puromycin and actinomycin. Biochem J. 1968 Feb;106(3):667–676. doi: 10.1042/bj1060667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wing D. R., Salaman M. R., Robinson D. S. Clearing-factor lipase in adipose tissue. Factors influencing the increase in enzyme activity produced on incubation of tissue from starved rats in vitro. Biochem J. 1966 Jun;99(3):648–656. doi: 10.1042/bj0990648. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES