Table 3.
Cecal and liver lesion rate and lesion scores.
| Group | +Liver LS | +Cecal LS | Liver LS | Cecal LS | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | 0 | 1 | 2 | 3 | 4 | |||
| Experiment 1 | ||||||||||||
| Oral 50 k | 0/10 (0%) | 0/10 (0%) | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 |
| Oral 100 k | 0/10 (0%) | 0/10 (0%) | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 |
| Oral 200 k | 2/10 (20%) | 6/10 (60%)* | 8 | 2 | 0 | 0 | 0 | 4 | 6 | 0 | 0 | 0 |
| Cloacal 50 k | 2/10 (20%) | 4/10 (40%) | 8 | 2 | 0 | 0 | 0 | 5 | 5 | 0 | 0 | 0 |
| Cloacal 100 k | 4/10 (40%) | 7/10 (70%)* | 6 | 2 | 2 | 0 | 0 | 3 | 4 | 1 | 2 | 0 |
| Cloacal 200 k | 5/10 (50%)* | 9/10 (90%)* | 5 | 1 | 3 | 0 | 1 | 1 | 4 | 1 | 3 | 1 |
| NC | 0/10 (0%) | 0/10 (0%) | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 |
| Experiment 2 | ||||||||||||
| D10 | 6/10 (60%)* | 7/10 (70%)* | 4 | 3 | 3 | 0 | 0 | 3 | 3 | 2 | 2 | 0 |
| D168 | 0/10 (0%) | 5/10 (50%)* | 10 | 0 | 0 | 0 | 0 | 5 | 4 | 1 | 0 | 0 |
| NC | 0/10 (0%) | 0/10 (0%) | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 |
| Experiment 3 | ||||||||||||
| Vacc Cloacal 50 k | 2/10 (20%) | 6/10 (60%)* | 8 | 2 | 0 | 0 | 0 | 4 | 4 | 2 | 0 | 0 |
| Vacc Cloacal 100 k | 1/10 (10%) | 4/10 (40%) | 9 | 1 | 0 | 0 | 0 | 6 | 3 | 1 | 0 | 0 |
| Vacc Cloacal 200 k | 0/10 (0%) | 4/10 (40%) | 10 | 0 | 0 | 0 | 0 | 6 | 4 | 0 | 0 | 0 |
| PC | 8/10 (80%)* | 9/10 (90%)* | 2 | 7 | 1 | 0 | 0 | 1 | 6 | 2 | 1 | 0 |
| NC | 0/10 (0%) | 0/10 (0%) | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 |
| Experiment 4 | ||||||||||||
| d3 Vacc | 2/10 (20%) | 5/10 (50%)* | 8 | 2 | 0 | 0 | 0 | 5 | 3 | 2 | 0 | 0 |
| d14 Vacc | 1/10 (10%) | 4/10 (40%) | 9 | 1 | 0 | 0 | 0 | 6 | 3 | 1 | 0 | 0 |
| d3/14 Vacc | 0/10 (0%) | 4/10 (40%) | 10 | 0 | 0 | 0 | 0 | 6 | 4 | 0 | 0 | 0 |
| PC | 4/10 (40%) | 8/10 (80%)* | 6 | 1 | 3 | 0 | 0 | 2 | 3 | 3 | 2 | 0 |
| NC | 0/10 (0%) | 0/10 (0%) | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 |
*Indicates significant difference in morbidities (P < 0.05) as compared to the NC group with chi-square test. Experiment 1 group: Oral 50 k, oral infection at a dose of 5 × 104 JSYZ-D10 H. meleagridis/chicken; Oral 100 k, oral infection at a dose of 1 × 105 JSYZ-D10 H. meleagridis/chicken; Oral 200 k, oral infection at a dose of 2 × 105 JSYZ-D10 H. meleagridis /chicken; Intracloacal 50 k, intracloacal infection at a dose of 5 × 104 JSYZ-D10 H. meleagridis /chicken; Intracloacal 100 k, intracloacal infection at a dose of 1 × 105 JSYZ-D10 H. meleagridis /chicken; Intracloacal 200 k, intracloacal infection at a dose of 2 × 105 JSYZ-D10 H. meleagridis /chicken; NC, non-challenged control. Experiment 2 group: D10, intracloacal infection at a dose of 2 × 105 JSYZ-D10 H. meleagridis/chicken; D168, intracloacal infection at a dose of 2 × 105 JSYZ-D168 H. meleagridis/chicken; NC, non-challenged control. Experiment 3 group: Vacc Intracloacal 50 k, intracloacal immunization at a dose of 5 × 104 JSYZ-D168 H. meleagridis/chicken; Vacc Intracloacal 100 k, intracloacal immunization at a dose of 1 × 105 JSYZ-D168 H. meleagridis/chicken; Vacc Intracloacal 200 k, intracloacal immunization at a dose of 2 × 105 JSYZ-D168 H. meleagridis/chicken; PC, positive-challenged control; NC, non-challenged control. Experiment 4 group: d3 Vacc, intracloacal immunization at a dose of 2 × 105 JSYZ-D168 H. meleagridis/chicken at 3 days of age; d14 Vacc, intracloacal immunization at a dose of 2 × 105 JSYZ-D168 H. meleagridis/chicken at 14 days of age; d3/14 Vacc, intracloacal immunization at a dose of 2 × 105 JSYZ-D168 H. meleagridis/chicken at 3 and 14 days of age, respectively; PC, positive-challenged control; NC, non-challenged control.