Table 2.

Optimized conditions of the BVDV E2 cELISA.

Optimized dilutions and reaction conditions	E2 cELISA
Coating condition	0.25 μg/well in CBS
	4°C, 16 h
Blocking condition	5% skimmed milk
	37°C, 1 h
Untested sample and the HRP-conjugated mAb 3E6	1:2 and 1:200, respectively
	37°C, 1 h
Chromogenic substrate	100 μL
	RT, 10 mins