Fig. 3.

(A) Detection by reverse transcription polymerase chain reaction (RT-PCR) of watermelon mosaic virus (WMV) in cucurbit and tobacco plants inoculated with the pWMV-M infectious clone. Total RNAs, isolated from upper non-inoculated leaves of each plant, were analyzed at 30 dpi by RT-PCR. Lanes 1 to 4, mock (empty binary vector)-inoculated plants; lanes 5 to 8, pWMV-M infectious clone-inoculated plants; lanes 1 and 5, watermelon; 2 and 6, zucchini; 3 and 7, melon; 4 and 8, Nicotiana benthamiana. N, negative (healthy) control; P, positive control inoculated with original isolate; M, 100-bp ladder. (B) Filamentous virus particles from pWMV-M inoculated melon leaves.