Table 1.
Primers used to construct an infectious full-length cDNA clone of WMV
| Primer | Sequence (5′→3′)a | Tm (°C) | Target (amplicon size, bp) |
|---|---|---|---|
| WMV-1F | aaattaaaacaactcataaa | 45 | WMV-frg1 (3,040) |
| WMV-1R | TGATTATGAAAATTTTTGCAACGCTATGTTCCTTATTTATccacatttcgattcccttct | ||
| WMV-2F | ataaataaggaacatagcgt | 53 | WMV-frg2 (3,760) |
| WMV-2R | CATACCCAACTCCAAACATTGTCTCCTTGTGACCATCTGAagagttcgtgagttggcaga | ||
| WMV-3F | tcagatggtcacaaggagac | 53 | WMV-frg3 (3,371) |
| WMV-3Rb | CAGGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCtttttttttttttttttttt | ||
| pJL89-F1 | gggtcggcatggcatctcca | 63 | pJL89 (4,721) |
| pJL89-R1c | CGTTTGTTTTGTTTGATGTCTTTATGAGTTGTTTTAATTTcctctccaaatgaaatgaac | ||
| Detection primer | |||
| WMV-UNI-1F | CAGTTTGAATCATGGTACAGCGC | 55 | WMV (392) |
| WMV-UNI-1R | TGTGCTATTGCTTCTCTTGCCC | ||
a
Sequences in capital letters represent 40 bp of overlapping nucleotides among the watermelon mosaic virus (WMV) RNAs and the pJL89 vector.
b
Underlined sequences represent the overlapping region with pJL89 vector nucleotides.
c
Underlined sequences represent the overlapping region with WMV RNA nucleotides.