Fig. 2.

TaqMan probe-based real-time reverse transcription-quantitative PCR detection of virus sequence in mixture with host RNAs extracted from branch tissue. (A) Reverse transcription-polymerase chain reaction products were electrophoresed on a 1.5% agarose gel stained with ethidium bromide. The negative control (NC) consisted only of total RNA from the host plants. (B) Amplification curves from the Cy5 channel were plotted. From red to navy, each color gradient represents 10 times the dilution of the previous one. The regression lines were generated from each quantification cycle (Cq) value by its concentration. The incline informs the efficiency of the qPCR. CiLV-C, citrus leprosis virus; CPsV, citrus psorosis virus; RFU, relative fluorescence unit.