Fig. 3.

Multiplex assay using different fluorescent channels. (A) Both CiLV-C and CPsV set1 were mixed in the real-time reverse transcription-quantitative PCR reactant. Templates are marked on the top left corner. The CiLV-C signal was read from the Cy5 channel, whereas the CPsV signal was read from FAM. (B) Duplex assay of internal control. The positive control vector was left with an artificial sequence trace to discern itself from the virus. The HEX-tagged probe detected the artificial sequence in the engineered vector but not the native virus sequence. CiLV-C, citrus leprosis virus; CPsV, citrus psorosis virus; FAM, fluorescein phosphoramidite; HEX, hexachloro-fluorescein; RFU, relative fluorescence unit.