Table 2.

Biophysical properties of Kv7.2 and Kv7.3 mutant channels

	n	pA/pF at 0 mV	V½ (mV)	k	Iinst/Iss	Activation τ @0 mV (ms)	Deactivation τ @−120 mV (ms)
Nontransfected	5	0.50 ± 0.2	-	-	-	-
Kv7.2	25	31.4 ± 4.6	−20.1 ± 1.1	12 ± 0.5	0.03 ± 0.01	177.7 ± 10.2	43.9 ± 4.0
Kv7.2 A317T	15	138.7 ± 20.3*	−92.9 ± 3.6*	13.6 ± 1.9	0.81 ± 0.04*	131.3 ± 12.7*	100.8 ± 10.7*
Kv7.2 L318V	8	105.3 ± 16.6*	−58.3 ± 1.3*	11.9 ± 11.1	0.33 ± 0.03*	104.1 ± 9.8*	142.1 ± 15.7*
Kv7.2 + Kv7.3	20	109.1 ± 11.9	−27.4 ± 1.2	11.4 ± 0.6	0.01 ± 0.003	225.1 ± 17.6	22.3 ± 1.9
Kv7.2 + Kv7.2 A317T+Kv7.3	12	178.0 ± 25.0†	−36.7 ± 2.1†	13.1 ± 2.1	0.12 ± 0.03†	147.1 ± 9.6†	35.5 ± 2.2†
Kv7.2 + Kv7.2 L318V+Kv7.3	9	178.1 ± 26.3†	−37.5 ± 1.4†	12 ± 0.8	0.04 ± 0.01	77.0 ± 8.7†	29.2 ± 4.8
Kv7.3	8	25.1 ± 5.9	−31.1 ± 2.0	9.5 ± 1.2	-	93.0 ± 6.4	-
Kv7.3 A356T	8	0.4 ± 0.1‡	-	-	-	-	-
Kv7.3 + pcDNA3.1	6	24.7 ± 7.8	−34.4 ± 2.3	6.7 ± 0.7		-	-
Kv7.3 + Kv7.3 A356T	8	7.9 ± 2.7‡	−34.6 ± 1.2	6.5 ± 0.7	-	111.5 ± 18.8	-
Kv7.2 + Kv7.3 A356T	9	371.9 ± 64.9†	−41.6 ± 3.7†	12 ± 0.7	0.08 ± 0.03†	109.7 ± 9.0†	29.5 ± 2.7
Kv7.2 + Kv7.3 + Kv7.3 A356T	9	261.6 ± 50.3†	−44.1 ± 1.2†	11.8 ± 1.2	0.05 ± 0.01†	86.2 ± 8.4†	24.6 ± 6.3

^* P < 0.05 versus Kv7.2.

^† P < 0.05 versus Kv7.2 + Kv7.3.

^‡ P < 0.05 versus Kv7.3.