Abstract
The relative binding affinity of Zn2+ to several glycosaminoglycans was determined by gel-filtration chromatography. Binding was observed only between Zn2+ and heparin. No binding was observed between Zn2+ and chondroitin 4-sulphate, chondroitin 6-sulphate, dermatan sulphate of hyaluronic acid. All of the glycosaminoglycans contained carboxy groups, but only heparin bound Zn2+. This observation suggests that, contrary to a previously proposed hypothesis, simple electrostatic interactions between the negatively charged carboxy groups of the glycosaminoglycans and the positively charged Zn2+ cannot explain the observed binding.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
- Fair W. R., Couch J., Wehner N. Prostatic antibacterial factor. Identity and significance. Urology. 1976 Feb;7(2):169–177. doi: 10.1016/0090-4295(76)90305-8. [DOI] [PubMed] [Google Scholar]
- Heathcote J. G., Washington R. J. Analysis of the zinc-binding protein derived from the human benign hypertrophic prostate. J Endocrinol. 1973 Sep;58(3):421–423. doi: 10.1677/joe.0.0580421. [DOI] [PubMed] [Google Scholar]
- Johnson L., Wikström S., Nylander G. The vehicle for zinc in the prostatic secretion of dogs. Scand J Urol Nephrol. 1969;3(1):9–11. doi: 10.3109/00365596909135373. [DOI] [PubMed] [Google Scholar]
- MAWSON C. A., FISCHER M. I. The occurrence of zinc in the human prostate gland. Can J Med Sci. 1952 Aug;30(4):336–339. doi: 10.1139/cjms52-043. [DOI] [PubMed] [Google Scholar]
- MAWSON C. A., FISCHER M. I. Zinc and carbonic anhydrase in human semen. Biochem J. 1953 Nov;55(4):696–700. doi: 10.1042/bj0550696. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reed M. J., Stitch S. R. The uptake of testosterone and zinc in vitro by the human benign hypertrophic prostate. J Endocrinol. 1973 Sep;58(3):405–419. doi: 10.1677/joe.0.0580405. [DOI] [PubMed] [Google Scholar]
- Sato C. S., Gyorkey F. Glycosaminoglycans of normal human prostate. Proc Soc Exp Biol Med. 1972 May;140(1):79–83. doi: 10.3181/00379727-140-36398. [DOI] [PubMed] [Google Scholar]
- Sato C. S., Györkey F. Further observations on the in vitro 65Zn-binding sites of human prostatic tissues. Cancer Res. 1969 Feb;29(2):399–402. [PubMed] [Google Scholar]