Figure 1. General timeline of CAM assay development in dental research during time.

Since 1980, CAM assay started to be used for dental research by using embryonic developing dental tissues to study tooth morphogenesis. Later, Greenberg proved the utility of CAM assay and its properties as a valuable platform for enamel differentiation. These preliminary studies were followed by CAM assay use for toxicological tests and also for the assessment of the angiogenic potential of various materials used in dentistry clinical practice. Recently, a combination of CAM assay and tooth organoids was published by Hermans et al. as an in vivo alternative to in vitro studies using organoids. This is an original figure created by the authors themselves. Figure 1 is created using special software (https://www.biorender.com/) dedicated to the graphical collage which may be inserted in the research articles. This service provides originality certificate for each created picture and also it gives us permission for publication.

CAM: Chorioallantoic membrane