Figure 5.

Effect of PGRN expression on subcutaneously transplanted tumor growth in nude mice. A. The transplanted tumors in the PGRN-shRNA transfected group was smaller than those in the empty vector-transfected group. B, C. The average weight of transplanted tumors in the PGRN-shRNA transfected group was significantly lower than that in the empty vector-transfected group, and the growth curve showed a delay (P < 0.01). D. Western blotting analysis of related proteins in both groups showed that the protein expression of MMP-2, MMP-9, and vimentin decreased, whereas P21 and E-cadherin expression increased in the PGRN-shRNA transfected group compared with those in the empty vector-transfected group (all P < 0.001) (n = 3). These results confirm that inhibiting PGRN expression can suppress tumor formation in gastric cancer cells in vivo.