Skip to main content
. 2024 Nov 13;27(12):111371. doi: 10.1016/j.isci.2024.111371

Figure 1.

Figure 1

Inducible inhibitory DREADDs attenuate cortical neuron excitability

Fluorescent images of cortical slices with neurons transduced with AAV2-TetOn and HiRet-TRE-hM4Di-mCherry. Example of recorded cortical neuron that expressed hM4Di-mCherry; (A) biocytin, (B) mCherry and (C) merged. Example of recorded cortical neuron (arrowhead) that did not express hM4Di-mCherry; (D) biocytin, (E) mCherry and (F) merged. Recorded neurons showing attenuation upon CNO application in neuronal excitability were hM4Di+/biocytin+. Recorded neurons that showed no CNO-mediated attenuation were hM4Di-/biocytin+.

(G) Change in number of spikes measured during electrical excitability of whole-cell patch clamped cortical neurons expressing hM4Di. Two-tailed unpaired t-test was significant: t(7.529, 6); p = 0.0003.

(H) Representative electrophysiology traces from a recorded hM4Di+ cortical neuron. Normal cell firing was observed during baseline measurements. CNO application temporarily attenuated cell firing that returned to normal upon washing out cell with ACSF. Data is mean ± SD. N = 2–6. ∗∗∗p < 0.001. Scale bars in (A) and (D) are 50 μm.