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. 2024 Nov 13;27(12):111371. doi: 10.1016/j.isci.2024.111371

Figure 2.

Figure 2

Neuronal silencing of C3-C4 PNs and/or RSNs in normal rats does not affect forelimb reaching behavior

(A) Silencing of C3/C4 PNs or RSNs was mediated by a 2 viral vector system for doxycycline inducible expression of hM4Di. For this study HiRet-tre-hM4Di-mCherry was injected into laminae VII of the C6-T1 spinal cord where it is preferentially taken up at synapses and retrogradely transported to multiple neuronal populations innervating that region. AAV-TetOn was stereotaxically injected into either the red nucleus, C3/C4 spinal cord or both. Representative images showing labeling of C3 propriospinal neurons (B) or red nucleus (C). Silencing of C3/C4 propriospinal neurons (D), rubrospinal neurons (E), or both (F) in normal rats shows no change in pellet retrieval with the administration of CNO either before or after induction of hM4Di expression with doxycycline. Silencing of both C3/C4 propriospinal neurons and rubrospinal neurons (G) failed to show reductions in hand and digit dexterity before or after CNO administration. Repeated measures one-way ANOVA was found not significant in D) F(4.921, 39.37) = 2.368; p = 0.0578, n = 9; E) F(3.445, 20.67) = 0.9004; p = 0.4695, n = 7; and F) F (4.166, 29.16) = 2.008; p = 0.1172, n = 8 for each group. Data is mean ± SEM. Scale bars in panels B is 250 μm and panel C is 150um.