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. 2024 Nov 8;39(1):13–20. doi: 10.1177/19458924241276274

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© The Author(s) 2024

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Figure 3. — Common cold coronavirus 229E increases ISG mRNA expression significantly more in ALI cultures from patients with CRSwNP by RT-qPCR. Fold changes were calculated by subtracting the measured fold change of the infected ALIs from the averaged mock values for nonCRS and CRSnp, respectively (A). ALI pooled cultures from 5 control donors without CRS and 5 donors with CRSwNP were infected with 229E (MOI = 5) in triplicate ALI cultures and ISG mRNA fold changes were measured at 48-, 96- and 144-hours post infection (hpi) (B). * = P < 0.05; ** = P < 0.01; *** = P < 0.001; and **** = P < 0.0001; ns = not significant.