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. Author manuscript; available in PMC: 2024 Dec 9.
Published in final edited form as: J Neurosci Res. 2020 Aug 23;100(1):19–34. doi: 10.1002/jnr.24682

FIGURE 11.

FIGURE 11

MOP internalization after agonist treatment. HEK 293T cells expressing esMOP were plated in serum-free DMEM and treated with saline (control), Met-enkephalin (Met-enk;1 μM), etorphine (E; 1 μM), or morphine (M; 1 μM) for 1 hr (top row). A separate group was treated with naloxone (Nal; 1 μM) 15 min prior to agonist treatment (bottom row). Met-enkephalin and etorphine treatment caused robust MOP internalization (MOP-Flag = green, DAPI = blue) that was blocked by naloxone pretreatment. Morphine, a noninternalizing opioid agonist, failed to stimulate MOP internalization in these cells. Scale bar = 75 μm