Deduced amino acid sequence of hagfish PENKL1. The translated sequence of hagfish PENKL1 ORF is shown. The functional motifs are highlighted: signal peptide (purple), disulfide-bonded cysteine (blue), degenerate enkephalin (pink), dibasic cleavage site (blue), met-enkephalin (maroon). Boxed regions denote potential products that were synthesized and tested for activity, labeled in blue letters