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. Author manuscript; available in PMC: 2024 Dec 9.
Published in final edited form as: J Neurosci Res. 2020 Aug 23;100(1):19–34. doi: 10.1002/jnr.24682

FIGURE 9.

FIGURE 9

esMOP MAPK signaling following agonist binding. (a) esMOP expressing HEK293T cells were serum starved overnight, and then treated with 0–3 μM Met-enkephalin or morphine for 5 min. Cell extracts were assayed by dot blot for phospho-MAPK, and intensity measured and expressed as percent change from control. Data represent means ± standard deviation, n = 3. (b) esMOP expressing HEK293T cells were cultured as above and treated with putative hagfish opioid peptides from esPENKL1: Y7S: YGGFMRS, Y13V: YGGFMRRFFGVAV, Y12L: YGGFMRRVGGPL, Y7N: YGRFMSN (1 μM) or Met-Enkephalin (Met-Enk; 1 μM) for 5 min. Phospho-MAPK was assessed with Western Blot