Table 1. Comparison of collagen scaffolds and CMC-PEG gels.
Material | Origin | Preparation | Sterilization | Manipulation | Washability | Variability |
---|---|---|---|---|---|---|
Collagen scaffolds | Natural (bovine collagen) | Disintegration → freeze-drying → cross-linking → freeze-drying | Ethylene oxide or gamma irradiation | Tweezers | Continuous experiments possible–scaffolds are washable and transferrable | Variable–each material has slightly different pore distribution and orientation; an unknown number of cells successfully seeded in the scaffolds (cell losses due to cells partly flowing outside the scaffold) |
CMC-PEG gel | CMC–natural; PEG–synthetic |
Powder + H2O → 120-140 °C for 30 min | UV light | Weighing, wide-bore tip pipetting | Difficult to wash (all additives mostly remain in the gel) | Each material displays the same features; a known number of cells seeded into the gel |
Note:
Washability refers to the ability of materials to be washed after exposure to a substance, which is non-toxic for a short period of time but can be toxic after long-exposure (e.g., AlamarBlue).