Table 2. Min, max, and median values of metabolic activity of CLL cells cultured in 24× diluted CMC-PEG compared to CLL cells cultured conventionally.

Group	IGHV	TP53	NOTCH1	Patient ID	Min (%)	Max (%)	Median (%)	Median of a group (%)	Median of all (%)
Gr1	Unmut	Mut	Mut	Pt08	87.52 (D7)	160.04 (D2)	119.22	115.59	115.20
				Pt09	87.20 (D7)	129.69 (D1)	113.53
				Pt10	84.16 (D7)	142.67 (D1)	119.45
Gr2	Unmut	Mut	WT	Pt11	94.45 (D4)	154.08 (D7)	119.37	112.63
				Pt12	66.57 (D7)	121.40 (D4)	109.87
				Pt13	71.20 (D7)	150.57 (D2)	111.08
Gr3	Unmut	WT	Mut	Pt14	91.44 (D2)	156.10 (D4)	116.57	111.44
				Pt15	83.33 (D7)	165.61 (D1)	114.91
				Pt16	87.41 (D4)	149.85 (D4)	98.47
Gr4	Unmut	WT	WT	Pt17	73.02 (D7)	169.57 (D4)	137.27	126.54
				Pt18	ND	ND	ND
				Pt19	61.86 (D7)	150.59 (D4)	113.55
Gr5	Mut	WT	WT	Pt20	111.75 (D2)	149.02 (D7)	121.35	120.48
				Pt21	77.37 (D7)	157.81 (D2)	108.48
				Pt22	111.49 (D4)	143.47 (D7)	122.18

Note:

Unmut, unmutated; Mut, mutated; WT, wild type; D, day, ND, not determined (as the absolute RFU values were nearly zero in both materials). The calculations can be seen in the accompanying raw data files (https://doi.org/10.5281/zenodo.13933534). Each measurement (RFU value) was compared with the mean of RFUs of biological replicates (n = 3) of conventional culture measured in the same patient and same day. This resulted in three percentual comparisons with conventional culture for each day. The median for each patient is a median of all these comparisons for each biological replicate (n = 3) of all four days (i.e., median of 3 × 4 = 12 comparisons in total). Median of a group of three patients is a median of 3 × 3 × 4 = 36 comparisons in total. Median of all (i.e., all 15 patients and their three comparisons for each of 4 days) is a median of 15 × 3 × 4 = 180 comparisons in total.