Figure 7.

Effect of potassium depletion on the subcellular distribution of GFP-SLC26A7 in MDCK cells. (Left) Normal potassium (4 mEq/L). (Middle two) Low potassium (2 mEq/L) for 2 and 16 h. (Right) Very low potassium (0.5 mEq/L) for 16 h. As demonstrated, SLC26A7 is expressed predominantly in punctate intracellular structures in normal potassium media (left) but is detected predominantly in the plasma in low-potassium medium for 16 h (right two). Second from left shows significant cytoplasmic localization with little membrane expression after short (2 h) incubation in low-potassium medium. Red, phalloidin; green, SLC26A7-GFP.