Fig 4. HSP70 interacts with SVCV G3 protein.

(A) Co-immunoprecipitation were used to detect the interaction between HSP70 and G3. 293T cells were transfected with pEGFP-HSP70 and/or pcMV-Flag-G3. Then, the cells were collected 24 h post-transfection and subsequently subjected to co-immunoprecipitation assay using an anti-Flag antibody. (B) Pull down were used to detect the interaction between HSP70 and G3. His-G3 protein was incubated with GST-HSP70, and pull-down assays were conducted using anti-GST magnetic beads. The elution was subsequently analyzed by Western blotting. (C) Laser co localization were used to detect the interaction between HSP70 and G3. 293T cells were co-transfected with pEGFP-C1, pCMV-C-DsRed, pEGFP-HSP70, and pCMV-DsRed-G3, respectively. At 24 h post transfection, the cells were fixed, permeabilized, incubated with DAPI, and subsequently visualized using a confocal microscope. Scale bars, 20 μm.