Fig 2. SEOV-induced ISG expression in HUVEC is RIG-I-dependent.

CRISPR-modified HUVEC (Cas9 alone, RIG-I^-/-, MDA5^-/-, or both RIG-I-like receptors (RLR^-/-) were infected with SEOV at MOI 0.01. Cell lysates and supernatant were collected every 24 hours for four days. A) Cell lysates were run on SDS-PAGE and subjected to immunoblot analysis. Data shown are representative of three independent experiments. Densitometry quantification of immunoblots in S2 Fig. B) Supernatants of SEOV-infected cells were collected on day four and analyzed for cytokine and chemokine levels. Data represent three biological replicates averaged across technical replicates ± SD. Two-way ANOVA with multiple comparisons, * denotes adjusted p<0.05.