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. 2024 Nov 25;20(11):e1012728. doi: 10.1371/journal.ppat.1012728

Fig 7. High MOI infections drive RLR-dependent ISG expression in RLMVEC.

Fig 7

A) RLMVEC were infected with SEOV at the noted MOI 0.05–0.75 and cell lysates collected every 24 hours, as indicated. Cell lysates were subjected to immunoblot analysis. Mock-infected RLMVEC (-) treated with 100U/mL IFNβ 24 hours prior to harvest serves as a positive control. B) Cas9 scramble, RIG-I-/-, MDA5-/-, or RLR-/- RLMVEC were mock-infected (-) or infected with SEOV at either MOI 0.05 or 0.5 and harvested at the indicated times post-infection. Lysates were subjected to immunoblot analysis. Densitometry quantification of immunoblots in Fig S8. All data shown represent ≥3 independent experiments.